t24  (Mirus Bio)

Journal: Frontiers in Immunology

Article Title: Ribosome biogenesis programs define a three-gene RBscore with prognostic relevance in bladder cancer

doi: 10.3389/fimmu.2026.1810132

Figure Lengend Snippet: Experimental validation of RBscore signature gene expression. (A) RT-qPCR of PIN4, POP4, and PRKDC mRNA in SV-HUC-1 and T24 cells. (B) RT-qPCR of PIN4, POP4, and PRKDC mRNA in paired BLCA and adjacent normal tissues. (C) Western blot analysis and quantification of PIN4, POP4, and PRKDC protein in SV-HUC-1 and T24 cells. (D) Representative IHC images of PRKDC in BLCA and adjacent normal tissues. (E) Quantification of PRKDC IHC scores in BLCA and adjacent normal tissues. * P < 0.05, *** P < 0.001. BLCA, bladder cancer; RBscore, ribosome biogenesis related score; IHC, immunohistochemistry.

Article Snippet: Human bladder urothelial SV-HUC-1 cells (Procell, China) and bladder cancer T24 cells (Procell, China) were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin–streptomycin at 37 °C in a humidified incubator with 5% CO 2 .

Techniques: Biomarker Discovery, Gene Expression, Quantitative RT-PCR, Western Blot, Immunohistochemistry